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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was identified to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a crucial function in advertising cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two added splice variants were being described but in depth nucleotide sequence data is not nonetheless obtainable.
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Blocking peptides are peptides that bind particularly on the target antibody and block antibody binding. These peptide normally includes the epitope recognized because of the antibody.
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This information outlines more feasible optimization of mobile and immunoassay parameters to be certain the absolute best outcomes are...
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This tutorial outlines further more doable optimization of cellular and immunoassay parameters to ensure the very best success are...
Antibodies sure to the blocking peptide no longer bind into the epitope around the focus on protein. By evaluating the staining in the blocked antibody versus the antibody on your own, one can see which staining is distinct.
Focus on Information Cyclin E belongs towards the remarkably conserved cyclin household, whose customers are characterised by a extraordinary periodicity in protein abundance from the mobile cycle. Cyclins functionality as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which add to the temporal coordination of each mitotic occasion. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose exercise is necessary for cell cycle G1/S transition. This protein accumulates in the G1-S section boundary and is particularly degraded as cells development via S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...
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